The Molecular Mechanism of Circular RNA Molecule CircSETD3 in Gefitinib Acquired Resistance in Non-Small Cell Lung Cancer

To explore the molecular pathway of circular RNA molecule in gefitinib acquired resistance nonsmall cell lung cancer and to provide a new therapeutic direction for clinical treatment non-small cancer. The gefitinib-sensitive strain H292_S was selected construct resistant model H292_R. Also, overexpression group vector control transfected circSETD3; knockdown plasmid H292_R circSETD3 were prepared. degree response measured by Western blotting counting kit-8 method. sequencing method used screen differentially expressed cells real-time fluorescent quantitative polymerase chain reaction verify results; dual-luciferase report relationship between microRNA-520h. After drug-resistant processed gefitinib, expression phosphorylated epidermal growth factor receptor, phosphatidylinositol 3-kinase protein kinase B decreased significantly. expressions three RNAs has_circ_0000567, has_circ_0000655 has_circ_0006867, all up-regulated strains, differences statistically significant (p<0.05). had effect on reproduction ability (p<0.05); wild type into 293T together with microRNA-520h mimics fluorescence intensity significantly In addition, if luciferase reporter mutant (mutant) mimics, would not change could be directly bound